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multiscript high-capacity reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscript high-capacity reverse transcriptase
    Multiscript High Capacity Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscript+high+capacity+reverse+transcriptase/multiscript+high+capacity+reverse+transcriptase/pm39981650-138-8-13
    Average 90 stars, based on 1 article reviews
    multiscript high-capacity reverse transcriptase - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Life-long persistence of infectious Zika virus: inflammation and behavioral sequela
    Article Snippet: To eliminate potential genomic DNA contamination, the DNA-free Turbo kit (ThermoFisher, Carlsbad, CA) was used as per the manufacturer’s protocol. .. Reverse transcription was performed on 1 μg of total RNA, using Multiscript High Capacity Reverse Transcriptase (ThermoFisher, Carlsbad, CA), per the manufacturer’s protocol, using random primers. .. The resulting cDNA was diluted ten-fold with ultra-pure, nuclease-free water and stored at −20 °C prior to use in real-time Taqman PCR reactions (ThermoFisher, Carlsbad, CA).

    Article Title: Neonatal Zika virus infection causes transient perineuronal net degradation
    Article Snippet: Total RNA was extracted from brain tissues using Trizol (Invitrogen, Waltham, MA). .. After DNase treatment (DNA-free Turbo kit; ThermoFisher), 1 μg of total RNA was used for cDNA synthesis using Multiscript High Capacity Reverse Transcriptase (ThermoFisher) using random primers per manufacturer’s protocol. .. Gene expression assays were performed using a Viia7 real-time PCR machine (ThermoFisher) with QuantStudio software (ThermoFisher).

    Article Title: Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice
    Article Snippet: RNA concentration and purity were determined by spectrophotometry at 260 nm and 280 nm using a NanoDrop 1000 spectrophotometer (ThermoFisher). .. After DNase treatment of 1 μg of RNA (DNA-free Turbo kit, ThermoFisher), reverse transcription was performed to generate cDNA using Multiscript High Capacity Reverse Transcriptase (ThermoFisher) using random primers per manufacturer’s protocol. ..

    Article Title: Zika (PRVABC59) Infection Is Associated with T cell Infiltration and Neurodegeneration in CNS of Immunocompetent Neonatal C57Bl/6 Mice
    Article Snippet: To eliminate potential genomic DNA contamination, the DNA-free Turbo kit (ThermoFisher, Carlsbad, CA) was used as per the manufacturers protocol. .. Reverse transcription was performed on 1 μg of total RNA, using Multiscript High Capacity Reverse Transcriptase (ThermoFisher, Carlsbad, CA), per the manufacturer’s protocol, using random primers. .. The resulting cDNA was diluted ten-fold with ultra-pure water and stored at -20°C prior to use in real-time Taqman PCR reactions (ThermoFisher, Carlsbad, CA).

    Article Title: Neonatal Zika virus infection causes transient perineuronal net degradation
    Article Snippet: Total RNA was extracted from brain tissues using Trizol (Invitrogen, Waltham, MA). .. After DNase treatment (DNA-free Turbo kit; ThermoFisher), 1 μg of total RNA was used for cDNA synthesis using Multiscript High Capacity Reverse Transcriptase (ThermoFisher) using random primers per manufacturer’s protocol. .. Gene expression assays were performed using a Viia7 real-time PCR machine (ThermoFisher) with QuantStudio software (ThermoFisher).

    Article Title: Long-term persistence of infectious Zika virus: Inflammation and behavioral sequela in mice
    Article Snippet: To eliminate potential genomic DNA contamination, the DNA-free Turbo kit (ThermoFisher, Carlsbad, CA) was used as per the manufacturer’s protocol. .. Reverse transcription was performed on 1 μg of total RNA, using Multiscript High Capacity Reverse Transcriptase (ThermoFisher, Carlsbad, CA), per the manufacturer’s protocol, using random primers. .. The resulting cDNA was diluted ten-fold with ultra-pure, nuclease-free water and stored at -20°C prior to use in real-time Taqman PCR reactions (ThermoFisher, Carlsbad, CA).

    Article Title: CD4 and CD8 T cells mediate distinct lethal meningoencephalitis in mice challenged with Tacaribe arenavirus
    Article Snippet: To eliminate potential genomic DNA contamination, the DNA-free Turbo kit (ThermoFisher) was used as per the manufacturer's protocol. .. Reverse transcription was performed on 1 μg of total RNA, using Multiscript High Capacity Reverse Transcriptase (ThermoFisher), as per the manufacturer's protocol, using random primers. .. The resulting cDNA was diluted 10-fold with ultra-pure water and stored at −20 °C prior to use in real-time TaqMan PCR reactions (ThermoFisher).

    cDNA Synthesis:

    Article Title: Neonatal Zika virus infection causes transient perineuronal net degradation
    Article Snippet: Total RNA was extracted from brain tissues using Trizol (Invitrogen, Waltham, MA). .. After DNase treatment (DNA-free Turbo kit; ThermoFisher), 1 μg of total RNA was used for cDNA synthesis using Multiscript High Capacity Reverse Transcriptase (ThermoFisher) using random primers per manufacturer’s protocol. .. Gene expression assays were performed using a Viia7 real-time PCR machine (ThermoFisher) with QuantStudio software (ThermoFisher).

    Article Title: Neonatal Zika virus infection causes transient perineuronal net degradation
    Article Snippet: Total RNA was extracted from brain tissues using Trizol (Invitrogen, Waltham, MA). .. After DNase treatment (DNA-free Turbo kit; ThermoFisher), 1 μg of total RNA was used for cDNA synthesis using Multiscript High Capacity Reverse Transcriptase (ThermoFisher) using random primers per manufacturer’s protocol. .. Gene expression assays were performed using a Viia7 real-time PCR machine (ThermoFisher) with QuantStudio software (ThermoFisher).



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